期刊
ACS APPLIED MATERIALS & INTERFACES
卷 10, 期 43, 页码 36796-36804出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.8b15419
关键词
biofuel cells; electrochemical biosensor; miRNA; porous Fe3O4 nanoparticles; duplex specific nuclease
资金
- National Natural Science Foundation of China [81771929]
- Natural Science Foundation of Jiangsu Province for Excellent Young Scholars [BK20170087]
- Jiangsu Province Young Medical Personnel [QNRC2016149]
- Wuxi Key Clinical Specialty [ZDXK006]
Herein, we have developed an ultrasensitive self-powered biosensor for miRNA assay based on biofuel cells. The system is composed of indium tin oxide cathode and graphene oxide/gold nanoparticle/glucose oxidase anode. Redox probe of [Fe(CN)(6)](3-) is entrapped inside porous Fe3O4 nanoparticles by DNA. However, in the presence of target miRNA, hybridization reaction occurs between miRNA and DNA, which initiates the release of [Fe(CN)(6)](3-). Moreover, duplex specific nuclease is further employed to trigger target recycling amplification. As a result, much more redox probes are released and the open circuit voltage is significantly increased. A signal-on self-powered biosensor for miRNA quantification is thus developed. The detection range is from 10 aM to 10 fM; meanwhile, the limit of detection is as low as 1.4 aM, which is superior to that in most reported methods. Therefore, the proposed biosensor is expected to be a powerful point-of-care tool for miRNA diagnostics, which may have wide applications in the future.
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