期刊
BIOCHEMICAL ENGINEERING JOURNAL
卷 103, 期 -, 页码 185-192出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bej.2015.07.019
关键词
Biomedical; Biosynthesis; Control; Metabolite over production; Phenolic acid; Salvia miltiorrhiza
资金
- National Natural Science Foundation of China [81373908]
- Ph.D. Programs Foundation of Ministry of Education of China [20110204110028]
Salvia miltiorrhiza is an important traditional Chinese herb in China and some other Asian countries. Phenolic acids are bioactive compounds in S. miltiorrhiza, the biosynthesis of which shares the general phenylpropanoid pathway with flavonoids. Chalcone synthase (CHS) is the entry point enzyme of the flavonoid pathway and plays a crucial role in flavonoid biosynthesis. In this study, the transcript of CHS was suppressed through RNAi-mediated silencing, and salicylic acid (SA) was added to the CHS silencing lines and wild-type lines as elicitor to investigate effects of the genetic modification and/or elicitor treatment on the production of phenolic acids in S. miltiorrhiza hairy roots. The results showed that the silencing of CHS enhanced contents of phenolic acids and decreased the accumulation of total flavonoids. The contents of phenolic acids in the SA treated CHS silencing lines were much higher than that in the CHS silencing lines and the SA treated wild-type lines. Our results imply that the substrate flow is directed to the phenolic acids pathway when the flavonoid pathway is interrupted, and combining the genetic modification and elicitor treatment is a new way to improve the production of phenolic acids in S. miltiorrhiza hairy root cultures. (C) 2015 Published by Elsevier B.V.
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