期刊
ACS APPLIED MATERIALS & INTERFACES
卷 11, 期 4, 页码 4523-4530出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.8b16819
关键词
nitric oxide; antimicrobial; antithrombotic; antifouling; surface chemistry
资金
- National Institutes of Health [K25HL111213, R01HL134899]
- University of Georgia start-up funds
- ARCS Foundation of Atlanta
Two major challenges faced by medical devices are thrombus formation and infection. In this work, surface tethered nitric oxide (NO)-releasing molecules are presented as a solution to combat infection and thrombosis. These materials possess a robust NO release capacity lasting ca. 1 month while simultaneously improving the nonfouling nature of the material by preventing platelet, protein, and bacteria adhesion. NO's potent bactericidal function has been implemented by a facile surface covalent attachment method to fabricate a triple-action coating surface-immobilized S-nitroso-N-acetylpenicillamine (SIM-S). Comparison of NO loading amongst the various branching configurations is shown through the NO release kinetics over time and the cumulative NO release. Biological characterization is performed using in vitro fibrinogen and Staphylococcus aureus assays. The material with the highest NO release, SIM-S2, is also able to reduce protein adhesion by 65.8 +/- 8.9% when compared to unmodified silicone. SIM-S2 demonstrates a 99.99% (i.e., similar to 4 log) reduction for S. aureus over 24 h. The various functionalized surfaces significantly reduce platelet adhesion in vitro, for both NO-releasing and non-NO-releasing surfaces (up to 89.1 +/- 0.9%), demonstrating the nonfouling nature of the surface-immobilized functionalities. The ability of the SIM-S surfaces to retain antifouling properties despite gradual depletion of the bactericidal source, NO, demonstrates its potential use in long-term medical implants.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据