期刊
ACS APPLIED MATERIALS & INTERFACES
卷 11, 期 6, 页码 5804-5811出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.8b19076
关键词
lead ion; biodistribution; cell imaging; aggregation-induced emission; supramolecular assembly
资金
- National Natural Science Foundation of China [21874141, 21675161, 21621062, 21705154]
- Ministry of Science and Technology of China [2015CB856303]
- Chinese Academy of Sciences
- Youth Innovation Promotion Association CAS [2015027]
Metal-peptide interactions provide plentiful resource and design principles for developing functional bio-materials and smart sensors. Pb2+, as a borderline metal ion, has versatile coordination modes. The interference from competing metal ions and endogenous chelating species greatly challenges Pb2+ analysis, especially in complicated living biosystems. Herein, a biomimetic peptide-based fluorescent sensor GSSH-2TPE was developed, starting from the structure of a naturally occurring peptide glutathione. Lewis acid-base theory was employed to guide the molecular design and tune the affinity and selectivity of the targeting performance. The integration of peptide recognition and aggregation-induced emission effect provides desirable sensing features, including specific turn-on response to Pb2+ over 18 different metal ions, rapid binding, and signal output, as well as high sensitivity with a detection limit of 1.5 nM. Mechanism investigation demonstrated the balance between the chelating groups, and the molecular configuration of the sensor contributes to the high selectivity toward Pb2+ complexation. The ion-induced supramolecular assembly lights up the bright fluorescence. The ability to image Pb2+ in living cells was exhibited with minimal interference from endogenous biothiols, no background fluorescence, and good biocompatibility. With good cell permeability, GSSH-2TPE can monitor changes in Pb2+ levels and biodistribution and thus predict possible damage pathways. Such metal-peptide interaction-based sensing systems offer tailorable platforms for designing bioanalytical tools and show great potential for studying the cell biology of metal ions in living biosystems.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据