期刊
NATURE BIOMEDICAL ENGINEERING
卷 2, 期 12, 页码 930-941出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41551-018-0271-5
关键词
-
资金
- John A. Paulson School of Engineering and Applied Sciences at Harvard University
- Wyss Institute for Biologically Inspired Engineering at Harvard University
- Harvard Materials Research Science and Engineering Center [DMR-1420570]
- Defense Threat Reduction Agency (DTRA) from Los Alamos National Laboratory under a prime DTRA [312659, DE-AC52-06NA25396]
- National Center for Advancing Translational Sciences of the National Institutes of Health [UH3TR000522, 1-UG3-HL-141798-01]
- US Army Research Laboratory
- US Army Research Office [W911NF-12-2-0036]
- National Science Foundation under NSF [1541959]
Laboratory studies of the heart use cell and tissue cultures to dissect heart function yet rely on animal models to measure pressure and volume dynamics. Here, we report tissue-engineered scale models of the human left ventricle, made of nanofibrous scaffolds that promote native-like anisotropic myocardial tissue genesis and chamber-level contractile function. Incorporating neonatal rat ventricular myocytes or cardiomyocytes derived from human induced pluripotent stem cells, the tissue-engineered ventricles have a diastolic chamber volume of similar to 500 mu l (comparable to that of the native rat ventricle and approximately 1/250 the size of the human ventricle), and ejection fractions and contractile work 50-250 times smaller and 104-108 times smaller than the corresponding values for rodent and human ventricles, respectively. We also measured tissue coverage and alignment, calcium-transient propagation and pressure-volume loops in the presence or absence of test compounds. Moreover, we describe an instrumented bioreactor with ventricular-assist capabilities, and provide a proof-of-concept disease model of structural arrhythmia. The model ventricles can be evaluated with the same assays used in animal models and in clinical settings.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据