期刊
BIOCHEMICAL ENGINEERING JOURNAL
卷 99, 期 -, 页码 1-15出版社
ELSEVIER
DOI: 10.1016/j.bej.2015.02.034
关键词
Enzymes; Biocatalysis; Kinetic parameters; Modeling; Laccase; Phenol
资金
- Natural Sciences and Engineering Research Council of Canada
- James McGill Chair award from McGill University
Oxidoreductase enzymes have the potential to be used to catalyze the oxidation of aromatic trace contaminants in wastewaters. The feasibility of this approach can be explored by modeling transient kinetics, particularly in the low substrate concentration range, where the model may be used to predict the quantity of enzyme and time required to achieve a particular level of conversion of a target compound. Laccase from Trametes versicolor was selected as a candidate enzyme due to its wide substrate specificity and its use of molecular oxygen as an oxidant. Phenol was selected as a target substrate. A four-parameter kinetic model was developed based on the known reactions of laccase. The model was applied to 30 sets of data collected from batch reactions conducted at pH 5 and 25 degrees C over a three hour period. Initial phenol concentrations ranged from 0.5 to 50 mu M and applied enzyme concentrations ranged from 0.12 to 2.5 mu M. The model demonstrated its utility for predicting the quantities of enzyme and reaction times required to achieve desired levels of oxidation of phenol for varying initial concentrations. A three-parameter simplified version of the kinetic model was also developed to facilitate calibration and mathematical solution of its equations. (C) 2015 Elsevier B.V. All rights reserved.
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