期刊
MICROSCOPY
卷 65, 期 1, 页码 3-8出版社
OXFORD UNIV PRESS
DOI: 10.1093/jmicro/dfv358
关键词
3D reconstruction; image processing; ribosome; molecular structure
类别
资金
- HHMI
- NIH [R01 GM 29169]
This is a brief account of the earlier history of single-particle cryo-EM of biological molecules lacking internal symmetry, which goes back to the mid-seventies. The emphasis of this review is on the mathematical concepts and computational approaches. It is written as the field experiences a turning point in the wake of the introduction of digital cameras capable of single electron counting, and near-atomic resolution can be reached even for smaller molecules.
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