期刊
RICE SCIENCE
卷 18, 期 4, 页码 265-272出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S1672-6308(12)60004-3
关键词
rice; plant height; QTL mapping; recombination inbred line; simple sequence repeat; sample size
资金
- Chinese Natural Science Foundation [31071398]
- National Program on Super Rice Breeding
- Ministry of Agriculture [2010-3]
- National High Technology Research and Development Program of China [2006AA10Z1E8]
- Provincial Program of '8812', Zhejiang Province, China [8812-1]
To clarify the most appropriate sample size for obtaining phenotypic data for a single line, we investigated the main-effect QTL (M-QTL) of a quantitative trait plant height (ph) in a recombinant inbred line (RIL) population of rice (derived from the cross between Xieqingzao B and Zhonghui 9308) using five individual plants in 2006 and 2009. Twenty-six ph phenotypic datasets from the completely random combinations of 2, 3, 4, and 5 plants in a single line, and five ph phenotypic datasets from five individual plants were used to detect the QTLs. Fifteen M-QTLs were detected by 1 to 31 datasets. Of these, qph7a was detected repeatedly by all the 31 ph datasets in 2006 and explained 11.67% to 23.93% of phenotypic variation; qph3 was detected repeatedly by all the 31 datasets and explained 5.21% to 7.93% and 11.51% to 24.46% of phenotypic variance in 2006 and 2009, respectively. The results indicate that the M-QTL for a quantitative trait could be detected repeatedly by the phenotypic values from 5 individual plants and 26 sets of completely random combinations of phenotypic data within a single line in an RIL population under different environments. The sample size for a single line of the RIL population did not affect the efficiency for identification of stably expressed M-QTLs.
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