期刊
BIOCHEMICAL ENGINEERING JOURNAL
卷 104, 期 -, 页码 74-81出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bej.2015.04.023
关键词
Packed bed bioreactors; Immobilized enzymes; Enzyme technology; Sucrose; Continuous operation; Silane-coated silica carriers
资金
- Fundacao para a Ciencia e a Tecnologia [SFRH/74818/2010]
A continuous flow enzyme microreactor was designed, assembled and run that allowed for operation with immobilized enzymes in particulate form. As a proof-of-concept, invertase was covalently bound to silane-coated silica carriers and used for the hydrolysis of sucrose into invert sugar syrup. Once glutaraldehyde solution and enzyme load were optimized, and the kinetic behavior of the immobilized biocatalyst was established, the particles were loaded in the sandwich type microreactor, resulting in a packed bed form. The microreactor was fed with substrate solution within 1.0-8.0% (w/v), at flow rates ranging from 17.5 to 259.0 mu L/min. Apparent kinetics were evaluated using the Lilly-Hornby model. Accordingly K-m(app) values decreased with increasing flow rates, whereas, for higher flow rates, the K-m(app) tends to stabilize at values close to that observed for the enzyme in the free form. Full conversion was observed up to 8.0% (w/v) of sucrose. Moreover, the immobilized invertase formulation packed in the microreactor displayed high operational stability, as it retained roughly 100% of its initial activity during 30 days. (C) 2015 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据