4.4 Review

Oligonucleotide primers, probes and molecular methods for the environmental monitoring of methanogenic archaea

期刊

MICROBIAL BIOTECHNOLOGY
卷 4, 期 5, 页码 585-602

出版社

WILEY
DOI: 10.1111/j.1751-7915.2010.00239.x

关键词

-

资金

  1. Environment Research and Technology Development Fund [S2-03]
  2. Ministry of the Environment, Japan [RF-076]

向作者/读者索取更多资源

For the identification and quantification of methanogenic archaea (methanogens) in environmental samples, various oligonucleotide probes/primers targeting phylogenetic markers of methanogens, such as 16S rRNA, 16S rRNA gene and the gene for the alpha-subunit of methyl coenzyme M reductase (mcrA), have been extensively developed and characterized experimentally. These oligonucleotides were designed to resolve different groups of methanogens at different taxonomic levels, and have been widely used as hybridization probes or polymerase chain reaction primers for membrane hybridization, fluorescence in situ hybridization, rRNA cleavage method, gene cloning, DNA microarray and quantitative polymerase chain reaction for studies in environmental and determinative microbiology. In this review, we present a comprehensive list of such oligonucleotide probes/primers, which enable us to determine methanogen populations in an environment quantitatively and hierarchically, with examples of the practical applications of the probes and primers.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.4
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据