期刊
MICROBIAL BIOTECHNOLOGY
卷 4, 期 6, 页码 777-793出版社
WILEY
DOI: 10.1111/j.1751-7915.2011.00281.x
关键词
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资金
- State Key Basic Research and Development Project of China [2011CB16141]
- Natural Science Foundation of China [30710103902, 31071656]
- National Transgenic Major Program [2008ZX08001-002]
- Special Fund for Agro-scientific Research in the Public Interest [NYHYZX07-056, 201003067-09]
Discoveries about antimicrobial peptides and plant defence activators have made possible the de novo and rational design of novel peptides for use in crop protection. Here we report a novel chimeric protein, Hcm1, which was made by linking the active domains of cecropin A and melittin to the hypersensitive response (HR)-elicitor Hpa1 of Xanthomonas oryzae pv. oryzicola, the causal agent of rice bacterial leaf streak. The resulting chimeric protein maintained not only the HR-inducing property of the harpin, but also the antimicrobial activity of the cecropin A-melittin hybrid. Hcm1 was purified from engineered Escherichia coli and evaluated in terms of the minimal inhibitory concentration (MIC) and the 50% effective dose (ED50) against important plant pathogenic bacteria and fungi. Importantly, the protein acted as a potential pesticide by inducing disease resistance for viral, bacterial and fungal pathogens. This designed drug can be considered as a lead compound for use in plant protection, either for the development of new broad-spectrum pesticides or for expression in transgenic plants.
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