Electrochemical behavior of paclitaxel and its determination at glassy carbon electrode

The electrochemical behavior of paclitaxel drug was studied at a glassy carbon electrode in phosphate buffer solutions using cyclic and differential-pulse voltammetric techniques. The oxidation process was shown to be irreversible over the pH range (3.0-10.4) and was diffusion controlled. Effects of anodic peak potential (E-p), anodic peak current (I-pa), scan rate, pH, heterogeneous rate constant (k(0)), etc have been discussed. A possible electro-oxidation mechanism was proposed. An analytical method was developed for the determination of paclitaxel in phosphate buffer solution at pH = 7.0 as a supporting electrolyte. The anodic peak current varied linearly with paclitaxel concentration in the range 1.0 x 10(-6) M to 1.0 x 10(-5) M with a limit of detection (LOD) of 1.23 x 10(-8) M and limit of quantification (LOQ) of 4.10 x 10(-8) M. The proposed method was successfully applied to the determination of paclitaxel in pure and real samples. (C) 2013 Shenyang Pharmaceutical University. Production and hosting by Elsevier B.V. All rights reserved.