期刊
ACTA PHARMACEUTICA SINICA B
卷 3, 期 1, 页码 55-64出版社
INST MATERIA MEDICA, CHINESE ACAD MEDICAL SCIENCES
DOI: 10.1016/j.apsb.2012.11.001
关键词
Rehmannia root; Liquid chromatography; Mass spectrometry; Evaporated light scattering detection; Multivariate statistical analysis
资金
- National Nature Science Foundation of China [81073161, 81130067, 30730112]
- National Basic Research Program of China [2011CB505304]
- Natural Science Foundation of Beijing [7112110]
To identify the chemical differences which lead to the different therapeutic effects of dried rehmannia root (DRR) and prepared rehmannia root (PRR), we compared the chemical composition of decoctions of randomly purchased DRR and PRR using ultra performance liquid chromatography (UPLC) coupled with time-of-flight mass spectrometry and high performance liquid chromatography (HPLC) coupled with evaporative light scattering detection (ELSD) with the aid of multivariate statistical analysis. Both approaches clearly revealed compositional and quantitative differences between DRR and PRR. UPLC-MS data indicated stachyose, rehmaionoside A (or rehmaionoside B), acteoside (or forsythiaside, or isoacteoside), 6-O-coumaroylajugol (or 6-O-E-feruloylajugol, or 6-O-Z-feruloylajugol) as important discriminators between DRR and PRR decoctions. HPLC-ELSD analysis showed that the content of fructose in the decoctions of PRR was about four times greater than that of DRR (P< 10(-5)), while sucrose content in the decoctions of PRR was only about one seventh of that in DRR (P<0.01). Our results suggest that some compounds, such as fructose, stachyose and rehmaionoside, may be responsible for the differing therapeutic effects of DRR and PRR. Furthermore, improvements in quality control for PRR, which is currently lacking in the Chinese Pharmacopoeia, are recommended. (C) 2012 Institute of Materia Medica, Chinese Academy of Medical Sciences and Chinese Pharmaceutical Association. Production and hosting by Elsevier B.V. All rights reserved.
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