期刊
EBIOMEDICINE
卷 35, 期 -, 页码 87-96出版社
ELSEVIER
DOI: 10.1016/j.ebiom.2018.08.028
关键词
Helicobacter pylori; Gut microbiota; 16S rRNA sequencing; Clostridium butyricum; Bismuth-containing quadruple therapy
资金
- Natural Science Foundation of Zhejiang Province [LY18H160011]
- Science and Technology Plan of Medicine and Health of Zhejiang Province [2017KY398]
- Science Foundation of Zhejiang Traditional Medicine Bureau [2017ZB063]
- Public Welfare Technology Application Research Plan of Zhejiang Province for Laboratory Animals [2017C37149]
- National Institutes of Health (NIH) [KL2TR002241]
Background: Helicobacter pylori (H. pylori) infection is associated with remodeling of gastric microbiota. However, comprehensive analyses of the impact of H. pylori infection, eradication therapy and probiotic supplementation on gut microbiota arc still lacking. We aimed to provide evidence for clinical decision making. Methods: Seventy H. pylon-positive and 35 H. pylori-negative patients (group C) were enrolled. It pylori-positive patients were randomly assigned to group A (14-day bismuth-containing quadruple therapy) and group B (quadruple therapy supplemented with Clostridium butyricum). Stool samples of group A and B were collected on day 0, 14 and 56 while stool samples of group C were collected on day O. Gut microbiota was investigated by 16S rRNA sequencing. Findings: The Sobs index (richness estimator) was significantly higher in H. pylori-positive samples than H. pylorinegative samples (p < .05). Several metabolic pathways were more abundant in H. pylon-positive communities while some disease-associated pathways had higher potential in H. pylori-negative community through KEGG pathway analysis. Abundances of most butyrate-producing bacteria significantly decreased, while several detrimental bacteria increased after eradication therapy. Probiotic supplementation was associated with improved gastrointestinal symptoms as well as increased Bucteroidetes:Firmicutes ratio. Interpretation: While H. pylori infection may not be necessarily detrimental in all patients, eradication of H. pylori was associated with widespread changes in gut microbial ecology and structure. Probiotic supplementation could relieve more gastrointestinal symptoms by inducing alterations in gut microbiota and host immune responses. As such, the decision to eradicate H. pylori should be based on comprehensive analysis of individual patients. (C) 2018 The Authors. Published by Elsevier B.V.
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