期刊
EBIOMEDICINE
卷 1, 期 1, 页码 72-79出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ebiom.2014.09.001
关键词
Vein conduits; Vascular diseases; Tissue-engineering; Endothelial precursors
资金
- Swedish Government LUA ALF grants to SSH andMO
- Swedish Heart and Lung Foundation [20130505]
- Swedish Research Council [K2013-65X-22347-01-3]
- Inga Britt and Arne Lundberg's Foundation [2009-362]
Vascular diseases are increasing health problems affecting >25 million individuals in westernized societies. Such patients could benefit from transplantation of tissue-engineered vascular grafts using autologous cells. One challenge that has limited this development is the need for cell isolation, and risks associated with ex vivo expanded stem cells. Here we demonstrate a novel approach to generate transplantable vascular grafts using decellularized allogeneic vascular scaffolds, repopulated with peripheral whole blood (PWB) in vitro in a bioreactor. Circulating, VEGFR-2+/CD45+ and a smaller fraction of VEGFR-2+/CD14+ cells contributed to repopulation of the graft. SEM micrographs showed flat cells on the luminal surface of the grafts consistent with endothelial cells. For clinical validation, two autologous PWBtissue-engineered vein conduits were prepared and successfully used for bypass procedures in two pediatric patients. These results provide a proof of principle for the generation of transplantable vascular grafts using a simple autologous blood sample, making it clinically feasible globally. (C) 2014 The Authors. Published by Elsevier B.V.
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