期刊
APPLIED BIOLOGICAL CHEMISTRY
卷 61, 期 6, 页码 599-606出版社
SPRINGER
DOI: 10.1007/s13765-018-0394-0
关键词
Biotin; Doxorubicin; Exosome; I-motif; pH-responsive; Streptavidin
资金
- Konkuk University
Nature-derived exosomes have been noted as emerging carriers for anticancer drugs. In this study, as a proof-of-concept, the anticancer drug doxorubicin (Dox) was loaded onto i-motif-modified exosomes (Exo-i-motif) to deliver Dox to cancer cells efficiently. The double-stranded biotin-i-motif/flare (ds-i-motif-bio)s efficiently released Dox in an acidic pH-responsive manner within 1h. Based on gel electrophoresis, it was clearly confirmed that ds-i-motif-bio successfully interacts with biotin-conjugated exosomes and streptavidin (strep) via the biotin-streptavidin interaction. The particle sizes were below 150nm without aggregation after strep-mediated modification of ds-i-motif-bio on the surfaces of the exosomes. In addition, released Dox had intact bioactivity for anti-proliferation after immobilization onto the exosomes. This study could serve as a new concept of pH-responsive delivery systems of anticancer drug using nature-derived exosomes with i-motifs.
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