期刊
MICROSCOPY
卷 63, 期 6, 页码 403-408出版社
OXFORD UNIV PRESS
DOI: 10.1093/jmicro/dfu033
关键词
diffusion coefficient; fluorescent protein; photobleaching; photoactivation; FRAP; FDAP
类别
资金
- MEXT [23115003, 23111502]
- JSPS [21687007]
- JST CREST
- JST PRESTO
- Grants-in-Aid for Scientific Research [21687007] Funding Source: KAKEN
Unlike in vitro protein dynamics, intracellular protein dynamics are intricately regulated by protein-protein interactions or interactions between proteins and other cellular components, including nucleic acids, the plasma membrane and the cytoskeleton. Alteration of these dynamics plays a crucial role in physiological phenomena such as gene expression and cell division. Live-cell imaging via microscopy with the inherent properties of fluorescent proteins, i.e. photobleaching and photoconversion, or fluorescence correlation spectroscopy, provides insight into the movement of proteins and their interactions with cellular components. This article reviews techniques based on photo-induced changes in the physicochemical properties of fluorescent proteins to measure protein dynamics inside living cells, and it also discusses the strengths and weaknesses of these techniques.
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