期刊
CHEMNANOMAT
卷 4, 期 12, 页码 1202-1208出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cnma.201800358
关键词
bifunctional nanoprobe; magnetite; europium; protein corona
资金
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES, Brazil)
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brazil)
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP, Brazil)
- INCT-Inomat
- Sistema Nacional de Laboratorios em Nanotecnologias (SisNANO)
The evaluation of the nanoparticles interactions with blood plasma enables a first and important insight on the organization of the adsorbed protein layer. In this context, we studied the formation of protein corona on magneto-luminescent Fe3O4@calix-Eu(TTA) nanoparticles in human plasma. The difference in the surface chemistry of F3O4 functionalized with calixarene (+30 mV zeta-potential) and europium (III) thenoyltrifluoroacetonate (Eu3+-TTA) complex (+7.4 mV zeta-potential) affected the colloidal stability and hard corona composition of the nanoparticles, which were monitored by SDS-PAGE gel electrophoresis, differential centrifugal sedimentation analyses and luminescence spectroscopy. Strikingly, after conjugation with Eu3+ TTA complex, the magnetic nanoparticles show lower adsorption affinity to proteins at higher blood plasma concentration. Moreover, the Eu3+ compound is featured with the narrow emission lines of D-5(0)-> F-7(J) (J=0-4) transitions, imparting additional characteristics to bifunctional nanoparticles that were used as probes to evaluate the protein corona formation.
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