期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 467, 期 2, 页码 400-406出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2015.09.138
关键词
Drosophila; Innate immunity; Sterile inflammation; Toll pathway; Danger signals
资金
- Japan Science and Technology Agency (JST)
- Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT)
- Program for the Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN)
- Global COE Research Grant (Tohoku University Ecosystem Adaptability)
- Takeda Science Foundation
- Kao Foundation for Arts and Sciences
- Uehara Memorial Foundation
- Futaba Electronics Memorial Foundation
- Grants-in-Aid for Scientific Research [15K18855] Funding Source: KAKEN
Damage-associated molecular patterns (DAMPs), so-called danger signals, play important roles in host defense and pathophysiology in mammals and insects. In Drosophila, the Toll pathway confers damage responses during bacterial infection and improper cell-fate control. However, the intrinsic ligands and signaling mechanisms that potentiate innate immune responses remain unknown. Here, we demonstrate that a Drosophila larva-derived tissue extract strongly elicits Toll pathway activation via the Toll receptor. Using this extract, we performed ex vivo genome-wide RNAi screening in Drosophila cultured cells, and identified several signaling factors that are required for host defense and antimicrobial-peptide expression in Drosophila adults. These results suggest that our larva-derived tissue extract contains active ingredients that mediate Toll pathway activation, and the screening data will shed light on the mechanisms of damage-related Toll pathway signaling in Drosophila. (C) 2015 Elsevier Inc. All rights reserved.
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