The longevity in the yeast Saccharomyces cerevisiae: A comparison of two approaches for assessment the lifespan

Longevity of the selected longevity mutants of yeast was studied using two methods. The standard method was based on counting the number of daughter cells produced. Modification of that method allowed for establishing the length of life expressed in units of time. It appeared that all the studied deletion longevity mutants showed a statistically meaningful increase in the number of daughters produced (replicative lifespan), whereas only one of the mutants, previously regarded as short lived, showed a meaningful increase in the time of life. The analysis of the available data shows that the time of life of most yeast strains is similar irrespective of their genetic background and mutations, which suggests a quasi-programmed nature of yeast death. (C) 2015 Elsevier Inc. All rights reserved.