期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 465, 期 2, 页码 293-298出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2015.08.027
关键词
MALAT1; Non-small cell lung cancer; TDP-43; Cellular migration and invasion
资金
- Tianjin Education Committee Foundation [20110101]
- National Natural Science Foundation of China [81201645, 30973383]
- Doctoral Program Foundation of Institutions of Higher Education [20121202120008]
- Key Project from National Natural Science Foundation of China [30430300]
- National 863 Program [2006AAOZA401]
- National 973 Program [2010CB529405]
- China-Sweden Cooperative Foundation [09ZCZDSF04100]
MALAT1 is a non-coding RNA overexpressed in non-small cell lung cancer (NSCLC). TDP-43 is a ubiquitously expressed, MALAT1-binding protein implicated in cancer development. We hypothesized that MALAT1 expression level is regulated in lung cancer by TDP-43. We analyzed their functions in cultured NSCLC cells. Downregulation of MALAT1 or TDP-43 expression by siRNA not only markedly suppressed NSCLC cell growth, as measured by the MTT assay in vitro cultured NSCLC cells (P < 0.05), but also noticeably impaired the migration and invasion of NSCLC cells, as analyzed by the migration and invasion assay. We also confirm that TDP-43 directly bound to MALAT1 RNA by a RNA immunoprecipitation (RIP) assay and by luciferase reporter activity assay. In a RT-PCR assay, silencing TDP-43 expression effectively decreased MALAT1 RNA transcript level. In contrast, TDP-43 overexpression markedly increased MALAT1 transcript level. In summary, these findings demonstrated that MALAT1 expression by regulation of TDP-43 controls cellular growth, migration, and invasion of NSCLCs. (C) 2015 Elsevier Inc. All rights reserved.
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