期刊
SURFACE INNOVATIONS
卷 1, 期 4, 页码 224-232出版社
ICE PUBLISHING
DOI: 10.1680/si.13.00019
关键词
adhesion; biomimetic; cell attachment; cell proliferation; gelatin; nitric oxide; surface modification
资金
- National Institute of Health (NIH)
- Young Clinical Scientist Award from Flight Attendance Medical Research Institute (FAMRI)
- Research Excellence Fund-Research Seed Grant (REF-RS) from Michigan Technological University
- Summer Undergraduate Research Fellowship (SURF) from Michigan Technological University
Nitric oxide plays important roles in cardiovascular homeostasis, immune responses and wound repair. Therefore, polymers that release nitric oxide locally at the surface exhibit improved biocompatibility for biomedical implants through reducing neointimal hyperplasia and thrombosis caused by blood vessel wall damage. The objective of this article was to fabricate a nitric oxide-releasing gelatin hydrogel that can continuously generate nitric oxide at a physiologically relevant level and inhibit cell attachment and proliferation. The nitric oxide donor, S-nitroso-N-acetylpenicillamine (SNAP), was successfully conjugated to the gelatin hydrogel, which showed a rapid nitric oxide release in the first 2 h and then a slower but sustained release in the next 70-h period. Human mesenchymal stem cells (hMSCs), as a model cell line with wide biomedical applications, were used to examine the cell attachment and proliferation of the nitric oxide-releasing gelatin hydrogel. Compared with the control gelatin, the nitric oxide-releasing gelatin hydrogel demonstrated a 0.35 times lower hMSCs attachment at 6 h and a 3.15 times lower hMSCs proliferation after 72-h incubation. Moreover, hMSCs on nitric oxide-releasing gelatin exhibited a rounder cell shape and covered less cellular area than their counterparts on the control gelatin hydrogel. This gelatin hydrogel with local nitric oxide release at physiological level provides a promising therapeutic approach in enhancing the performance of biomedical implants.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据