期刊
REDOX BIOLOGY
卷 2, 期 -, 页码 513-519出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.redox.2014.01.004
关键词
Airway epithelial cells; Air-liquid interface culture; Metabolism; Oxygen consumption rate (OCR); Extracellular acidification rate (ECAR)
资金
- American Asthma Foundation
- [HL081064]
- [HL103453]
- [HL107147]
- [HL109250]
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [P01HL081064, P01HL107147, P01HL103453, U10HL109250] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [P30DK027651] Funding Source: NIH RePORTER
Air-liquid interface cell culture is an organotypic model for study of differentiated functional airway epithelium in vitro. Dysregulation of cellular energy metabolism and mitochondria! function have been suggested to contribute to airway diseases. However, there is currently no established method to determine oxygen consumption and glycolysis in airway epithelium in air-liquid interface. In order to study metabolism in differentiated airway epithelial cells, we engineered an insert for the Seahorse XF24 Analyzer that enabled the measure of respiration by oxygen consumption rate (OCR) and glycolysis by extracellular acidification rate (ECAR). Oxidative metabolism and glycolysis in airway epithelial cells cultured on the inserts were successfully measured. The inserts did not affect the measures of OCR or ECAR. Cells under media with apical and basolateral feeding had less oxidative metabolism as compared to cells on the inserts at air-interface with basolateral feeding. The design of inserts that can be used in the measure of bioenergetics in small numbers of cells in an organotypic state may be useful for evaluation of new drugs and metabolic mechanisms that underlie airway diseases. (C) 2014 The Authors. Published by Elsevier B.V. All rights reserved.
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