期刊
CENTRAL EUROPEAN JOURNAL OF BIOLOGY
卷 9, 期 10, 页码 915-921出版社
DE GRUYTER POLAND SP ZOO
DOI: 10.2478/s11535-014-0326-x
关键词
Extremely high-frequency electromagnetic radiation; Pulse modulation; X-rays; Hydrogen peroxide; DNA damage; Comet assay; Protective effect
类别
资金
- Foundation for Assistance to Small Innovative Enterprises [10274p/18076]
- Russian Foundation for Basic Research [13-04-00730-a]
It is believed that non-ionizing electromagnetic radiation (EMR) and low-level hydrogen peroxide (H2O2) may change nonspecific resistance and modify DNA damage caused by ionizing radiation. To check this assumption, the combined effects of extremely high-frequency EMR (EHF EMR) and X-rays on induction of DNA damage in mouse whole blood leukocytes were studied. The cells were exposed to X-rays with or without preliminary treatment with EHF EMR or low-level H2O2. With the use of enhanced chemiluminescence, it was shown for the first time that pulse-modulated EHF EMR (42.2 GHz, incident power density of 0.1 mW/cm(2), exposure duration of 20 min, modulation frequency of 1 Hz) induced H2O2 at a concentration of 4.6 +/- 0.3 nM L-1 in physiological saline. With the use of an alkaline comet assay, it was found that the exposure of cells to the pulse-modulated EHF EMR, 25 min prior to treatment with X-rays at a dose of 4 Gy reduced the level of ionizing radiation-induced DNA damage. Continuous EHF EMR was inefficient. In turn, it was shown that low-level H2O2 (30-500 nM L-1) protected the cells against X-irradiation. Thus, the mechanisms of radiation protective effect of EHF EMR are connected with the induction of the adaptive response by nanomolar concentrations of reactive oxygen species formed by pulse-modulated EHF EMR.
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