期刊
CENTRAL EUROPEAN JOURNAL OF BIOLOGY
卷 7, 期 5, 页码 850-857出版社
DE GRUYTER POLAND SP Z O O
DOI: 10.2478/s11535-012-0071-y
关键词
Ascomycetes; Basidiomycetes; Endangered species; Fungal associates; Photosynthetic orchids
类别
资金
- Estonian Science Foundation
- European Social Fund [MJD135]
We have assessed the identities of fungi associated with Orchis tridentata, an endangered orchid species growing in open woodland and poor grassland of Central and Southern Europe. Fungal diversity in ten O. tridentata adult individuals collected in two protected areas of Central Italy was analysed by means of morphological and molecular methods. Sequencing of the cloned ITS fungal inserts corresponding to the dominant PCR products obtained from amplification of total root DNA with ITS1F and ITS4 primers revealed a variety of fungal species occurring in O. tridentata roots. Among them, members of the basidiomycete families Ceratobasidiaceae, Tulasnellaceae and Hymenogastraceae were recovered, together with ascomycetes belonging to Leptodontidium and Terfezia. The implications of these results in the understanding of O. tridentata biology and for the conservation of this threatened orchid species are discussed.
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