期刊
ONCOIMMUNOLOGY
卷 3, 期 6, 页码 -出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/onci.29256
关键词
colorectal cancer; microsatellite instability; inflammation; cytokine; chemokine
资金
- Societe Nationale Francaise de Gastroenterologie (SNFGE)
Sporadic or hereditary colorectal cancer (CRC) with microsatellite instability (MSI) is frequently characterized by inflammatory lymphocytic infiltration and tends to be associated with a better outcome than microsatellite stable (MSS) CRC, probably reflecting a more effective immune response. We investigated inflammatory mechanisms in 48 MSI CRCs and 62 MSS CRCs by analyzing: (1) the expression of 48 cytokines using Bio-Plex multiplex cytokine assays, and (2) the in situ immune response by immunohistochemical analysis with antibodies against CD3 (T lymphocytes), CD8 (cytotoxic T lymphocytes), CD45RO (memory T lymphocytes), T-bet (Th1 CD4 cells), and FoxP3 (regulatory T cells). MSI CRC exhibited significantly higher expression of CCL5 (RAN TES), CXCL 8 (IL-8), CXCL 9 (MIG), IL-1 beta, CXCL 10 (IP-10), IL-16, CXCL 1 (GRO alpha), and IL-1ra, and lower expression of MIF, compared with MSS CRC. Immunohistochemistry combined with image analysis indicated that the density of CD3(+), CD8(+), CD45RO(+), and T-bet(+) T lymphocytes was higher in MSI CRC than in MSS CRC, whereas the number of regulatory T cells (FoxP3(+)) was not statistically different between the groups. These results indicate that MSI CRC is associated with a specific cytokine expression profile that includes CCL5, CXCL 10, and CXCL 9, which are involved in the T helper type 1 (Th1) response and in the recruitment of memory CD45RO(+) T cells. Our findings highlight the major role of adaptive immunity in MSI CRC and provide a possible explanation for the more favorable prognosis of this CRC subtype.
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