期刊
ONCOIMMUNOLOGY
卷 1, 期 6, 页码 829-839出版社
LANDES BIOSCIENCE
DOI: 10.4161/onci.20492
关键词
tumor-infiltrating lymphocytes; blood vessels; melanoma; immunity; chemokine; dendritic cells
资金
- Fondation RITC
- Region Midi-Pyrenees
- GR-ICR
- Association pour la Recherche Contre le Cancer
- Ligue Nationale Contre le Cancer (Equipe Labelisee Ligue)
The presence of tumor-infiltrating lymphocytes (TILs) is a strong prognostic parameter for local dissemination and overall survival in melanoma. Lymphocyte migration from blood into peripheral tissues is mainly regulated by vascular endothelium. However, the blood vessels and mechanisms governing the recruitment of TILs in melanoma tumors remain poorly understood. Here, we show that high endothelial venules (HE Vs), specialized blood vessels for lymphocyte extravasation into lymphoid tissues, are frequently found in melanoma tumors and are associated with high levels of lymphocyte infiltration. The analysis of 225 primary melanomas revealed that lymphocytes specifically infiltrated HE V-rich areas of melanoma tumors and that the density of MECA -79(+) HEVs was variable among patients and strongly correlated with CD3(+), CD8(+) and CD20(+) TIL densities. Inflammatory (CCL5, CXCL9, CXCL10 and CXCL11) and lymphoid (CCL21, CCL19 and CXCL13) chemokines as well as T(H)1 and naive T-cell genes were overexpressed in melanoma samples with high densities of tumor HE Vs. Mature dendritic cells (mDCs) were frequently found around tumor HE Vs and densities of HE Vs and DC-LAMP(+) mDCs within tumor stroma were strongly correlated. DCs which maintain HE Vs in lymph nodes, may thus also contribute to the regulation of HE Vs in melanomas. Finally, we found significantly higher densities of tumor HE Vs in melanomas with tumor regression, low Clark level of invasion and thin Breslow thickness (all p < 0.001). The strong association between tumor HE Vs, TILs, mDCs and clinical parameters of melanoma, supports a critical role for HE Vs in limiting malignant melanoma development through both naive and effector T-lymphocyte recruitment and activation.
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