期刊
MICROBIOME
卷 2, 期 -, 页码 -出版社
BMC
DOI: 10.1186/2049-2618-2-14
关键词
Cystic fibrosis; Microbiome; Pseudomonas aeruginosa; Sputum
类别
资金
- Flatley Foundation of Boston
- National Institutes of Health (NIH) [P20 GM103413-10, R01 HL074175-09]
- Cystic Fibrosis Foundation Research Development Program grant [STANTO07R0, R025-CR07]
- NIH [R01 HL068927-09, R01 DK44003, R01 AI091699, 4UH3DK083993]
- Cystic Fibrosis Foundation [CUTTIN06P0]
Background: Cystic fibrosis (CF) is caused by mutations in the CFTR gene that predispose the airway to infection. Chronic infection by pathogens such as Pseudomonas aeruginosa leads to inflammation that gradually degrades lung function, resulting in morbidity and early mortality. In a previous study of CF monozygotic twins, we demonstrate that genetic modifiers significantly affect the establishment of persistent P. aeruginosa colonization in CF. Recognizing that bacteria other than P. aeruginosa contribute to the CF microbiome and associated pathology, we used deep sequencing of sputum from pediatric monozygotic twins and nontwin siblings with CF to characterize pediatric bacterial communities and the role that genetics plays in their evolution. Findings: We found that the microbial communities in sputum from pediatric patients living together were much more alike than those from pediatric individuals living apart, regardless of whether samples were taken from monozygous twins or from nontwin CF siblings living together, which we used as a proxy for dizygous twins. In contrast, adult communities were comparatively monolithic and much less diverse than the microbiome of pediatric patients. Conclusion: Taken together, these data and other recent studies suggest that as patients age, the CF microbiome becomes less diverse, more refractory to treatment and dominated by mucoid P. aeruginosa, as well as being associated with accelerated pulmonary decline. Our studies show that the microbiome of pediatric patients is susceptible to environmental influences, suggesting that interventions to preserve the community structure found in young CF patients might be possible, perhaps slowing disease progression.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据