期刊
SPRINGERPLUS
卷 4, 期 -, 页码 -出版社
SPRINGER INTERNATIONAL PUBLISHING AG
DOI: 10.1186/s40064-015-1432-6
关键词
Hydrolyzed proteins; Primary liver cells; Head kidney cells; Co-cultures; Oxidative stress; Viability; Caspase-3; Atlantic salmon
资金
- Research Council of Norway through the project Value creation through characterization of bioactive peptides in residues material from Atlantic salmon- An integrated approach [ES490421]
- Hordafor AS.
- Chinese 973 program [2014CB138603]
- NSF [31172419/C190401]
Hydrolyzed fish proteins (H-pro) contains high concentrations of free amino acids and low molecular peptides that potentially benefit health. The following study aimed to test whether the water soluble phase of H-pro could reduce apoptosis and inflammation in primary liver cells isolated from Atlantic salmon following H2O2 provoked oxidative stress. Cells were grown as monocultures or co-cultured with head kidney cells to assess possible cross talk in inflammation and metabolism during treatments. Cells were grown in media with or without H-pro for 2 days before being stressed with 200 mu M H2O2 then harvested 24 h post exposure. Both treatments were compared to the respective treatments without H2O2 supplementation. Oxidative stressed cells had increased activation of caspase-3, but supplementation with H-pro in the media prior to the oxidative stress reduced caspase-3 activation. In conclusion, free amino acids and low molecular weight peptides from H-pro attenuated oxidative stress, and made cells able to withstand apoptosis after H2O2 provoked oxidative stress.
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