A quantitative study on the formation of Pseudomonas aeruginosa biofilm

Biofilms are bacterial cells in a matrix of extracellular polymeric substance. The formation of biofilm depends on the microenvironment. In this study, the effect of temperature on Pseudomonas aeruginosa biofilm formation was evaluated with respect to three parameters-the mass of biofilm formed, the production of extracellular polysaccharide and the adhesion force. The results indicate that biofilm biomass (2.8, A590), extracellular polysaccharide production (1240 +/- 40 mu g) and adhesion force (10.8 +/- 0.2 nN) were highest at 37 degrees C. The results also suggest that biofilms formed at 37 degrees C would have a higher mechanical stability (than biofilms grown at 28, 33 and 42 degrees C).