期刊
JOURNAL OF SYNCHROTRON RADIATION
卷 25, 期 -, 页码 1362-1370出版社
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S1600577518010354
关键词
macromolecular crystallography; nano-positioning; serial crystallography; raster-scanning; high-precision goniometer
资金
- BNL LDRD program [16-006]
- Brookhaven National Laboratory [DE-SC0012704]
- US National Institutes of Health NIGMS [P41GM111244]
The Frontier Microfocus Macromolecular Crystallography (FMX) beamline at the National Synchrotron Light Source II with its 1 mu m beam size and photon flux of 3 x 10(12) photonss(-1) at a photon energy of 12.66keV has reached unprecedented dose rates for a structural biology beamline. The high dose rate presents a great advantage for serial microcrystallography in cutting measurement time from hours to minutes. To provide the instrumentation basis for such measurements at the full flux of the FMX beamline, a high-speed, high-precision goniometer based on a unique XYZ piezo positioner has been designed and constructed. The piezo-based goniometer is able to achieve sub-100nm raster-scanning precision at over 10 grid-linepairss(-1) frequency for fly scans of a 200 mu m-wide raster. The performance of the scanner in both laboratory and serial crystallography measurements up to the maximum frame rate of 750Hz of the Eiger 16M's 4M region-of-interest mode has been verified in this work. This unprecedented experimental speed significantly reduces serial-crystallography data collection time at synchrotrons, allowing utilization of the full brightness of the emerging synchrotron radiation facilities.
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