期刊
JOURNAL OF SYNCHROTRON RADIATION
卷 25, 期 -, 页码 1478-1489出版社
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S1600577518010093
关键词
X-ray tomography; image alignment; image reconstruction; parallelized computing
资金
- National Institute of Mental Health [U01-MH109100]
- US Department of Energy, Office of Science [DE-AC02-06CH11357]
X-rays offer high penetration with the potential for tomography of centimetre-sized specimens, but synchrotron beamlines often provide illumination that is only millimetres wide. Here an approach is demonstrated termed Tomosaic for tomographic imaging of large samples that extend beyond the illumination field of view of an X-ray imaging system. This includes software modules for image stitching and calibration, while making use of existing modules available in other packages for alignment and reconstruction. The approach is compatible with conventional beamline hardware, while providing a dose-efficient method of data acquisition. By using parallelization on a distributed computing system, it provides a solution for handling teravoxel-sized or larger datasets that cannot be processed on a single workstation in a reasonable time. Using experimental data, the package is shown to provide good quality three-dimensional reconstruction for centimetre-sized samples with sub-micrometre pixel size.
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