4.8 Article

Cultured Human Thymic-Derived Cells Display Medullary Thymic Epithelial Cell Phenotype and Functionality

期刊

FRONTIERS IN IMMUNOLOGY
卷 9, 期 -, 页码 -

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2018.01663

关键词

thymic epithelial cells; primary cell culture method; keratins; cytokines; chemokines; tissue-specific antigens

资金

  1. MYASTAID [LSHM-CT-2006-037833]
  2. FIGHT-MG from the European Community [HEALTH-2009-242-210]
  3. Agence Nationale de la Recherche [ANR-06-MRAR-001-01]
  4. Association Francaise contre les Myopathies

向作者/读者索取更多资源

Thymic epithelial cells are one of the main components of the thymic microenvironment required for T-cell development. In this work, we describe an efficient method free of enzymatic and Facs-sorted methods to culture human medullary thymic epithelial cells without affecting the cell phenotypic, physiologic and functional features. Human medulla thymic epithelial cells (mTECs) are obtained by culturing thymic biopsies explants. After 7 days of primo-culture, mTECs keep their ability to express key molecules involved in immune tolerance processes such as autoimmune regulator, tissue-specific antigens, chemokines, and cytokines. In addition, the cells sensor their cultured environment and consequently adjust their gene expression network. Therefore, we describe and provide a human mTEC model that may be used to test the effect of various molecules on thymic epithelial cell homeostasis and physiology. This method should allow the investigations of the specificities and the knowledge of human mTECs in normal or pathological conditions and therefore discontinue the extrapolations done on the murine models.

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