期刊
CLINICAL PROTEOMICS
卷 15, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12014-018-9204-2
关键词
Proteomics; Acinetobacter baumannii; Outer membrane vesicles; Modulation by antibiotic treatment
资金
- Korea Health Technology R&D Project through Korea Health Industry Development Institute (KHIDI) - Ministry of Health and Welfare
- National Research Council of Science and Technology (NST) grant by Korea government (MSIP) [CRC-16-01-KRICT]
- Korea Basic Science Institute research program [C38932]
- UST Young Scientist program 2018
Background: Outer membrane vesicles (OMVs) ofAcinetobacter baumannii are cytotoxic and elicit a potent innate immune response. OMVs were first identified in A. baumannii DU202, an extensively drug-resistant clinical strain. Herein, we investigated protein components of A. baumannii DU202 OMVs following antibiotic treatment by proteogenomic analysis. Methods: Purified OMVs from A. baumannii DU202 grown in different antibiotic culture conditions were screened for pathogenic and immunogenic effects, and subjected to quantitative proteomic analysis by one-dimensional electrophoresis and liquid chromatography combined with tandem mass spectrometry (1DE-LC-MS/MS). Protein components modulated by imipenem were identified and discussed. Results: OMV secretion was increased >twofold following imipenem treatment, and cytotoxicity toward A549 human lung carcinoma cells was elevated. A total of 277 proteins were identified as components of OMVs by imipenem treatment, among which beta-lactamase OXA-23, various proteases, outer membrane proteins, beta-barrel assembly machine proteins, peptidyl-prolyl cis-trans isomerases and inherent prophage head subunit proteins were significantly upregulated. Conclusion: In vitro stress such as antibiotic treatment can modulate proteome components in A. baumannii OMVs and thereby influence pathogenicity.
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