期刊
BIOENGINEERED
卷 4, 期 6, 页码 438-442出版社
LANDES BIOSCIENCE
DOI: 10.4161/bioe.24514
关键词
insect cell; recombinant protein production; virus-like particle; Japanese encephalitis virus; High Five
资金
- Japan Society for the Promotion of Science [22360345]
- Grants-in-Aid for Scientific Research [24656505, 22360345] Funding Source: KAKEN
Virus-like particles (VLPs) are composed of one or several recombinant viral surface proteins that spontaneously assemble into particulate structures without the incorporation of virus DNA or RNA. The baculovirus-insect cell system has been used extensively for the production of recombinant virus proteins including VLPs. While the baculovirus-insect cell system directs the transient expression of recombinant proteins in a batch culture, stably transformed insect cells allow constitutive production. In our recent study, a secretory form of Japanese encephalitis (JE) VLPs was successfully produced by Trichoplusia ni BTI-TN-5B1-4 (High Five) cells engineered to coexpress the JE virus (JEV) premembrane (prM) and envelope (E) proteins. A higher yield of E protein was attained with recombinant High Five cells than with the baculovirus-insect cell system. This study demonstrated that recombinant insect cells offer a promising approach to the high-level production of VLPs for use as vaccines and diagnostic antigens.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据