期刊
BIOENGINEERED
卷 3, 期 4, 页码 203-208出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/bioe.19567
关键词
polyhydroxyalkanoate; polyester; biopolymer; PHA; PHB; beads; vaccines; bioseparation; bacterial inclusions; PHA synthase
资金
- New Zealand Ministry of Science and Innovation, Massey University
- Polybatics Ltd.
The ability to generate tailor-made, functionalized polyester (polyhydroxyalkanoate, PHA) beads in bacteria by harnessing their natural carbon-storage granule production system is an exciting recent development. Proteins that naturally attach to the polyester granule core were rationally engineered to enable in vivo production of PHA beads which are applicable in bioseparation, protein purification, enzyme immobilization and diagnostics and which show advantageous properties toward the development of safe and efficient particulate vaccines. These beads are recombinantly produced as fully functional, insoluble polyester inclusions that can be easily separated from the cell. This simple one-step production of functionalized beads provides a tantalizing alternative to current commercial functional beads, for which proteins must be expressed, purified and then chemically attached to solid supports. The recent success in generating antigen-displaying PHA granules in the food-grade bacterium Lactococcus lactis capable of mediating protective immunity against Mycobacterium tuberculosis infection highlights the promise and flexibility of this new technology.
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