期刊
AMB EXPRESS
卷 2, 期 -, 页码 -出版社
SPRINGER
DOI: 10.1186/2191-0855-2-59
关键词
Poly (3-hydroxybutyrate); Polyhydroxyalkanoates; Ralstonia eutropha; PHA recovery; Sodium hypochlorite; Downstream process
资金
- King Abdulaziz University [2- 10- 1432/ HiCi]
- KAU
Isolation of polyhydroxyalkanoates (PHAs) from bacterial cell matter is a critical step in order to achieve a profitable production of the polymer. Therefore, an extraction method must lead to a high recovery of a pure product at low costs. This study presents a simplified method for large scale poly(3-hydroxybutyrate), poly(3HB), extraction using sodium hypochlorite. Poly(3HB) was extracted from cells of Ralstonia eutropha H16 at almost 96% purity. At different extraction volumes, a maximum recovery rate of 91.32% was obtained. At the largest extraction volume of 50 L, poly(3HB) with an average purity of 93.32% +/- 4.62% was extracted with a maximum recovery of 87.03% of the initial poly(3HB) content. This process is easy to handle and requires less efforts than previously described processes.
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