期刊
AMB EXPRESS
卷 2, 期 -, 页码 -出版社
BIOMED CENTRAL LTD
DOI: 10.1186/2191-0855-2-35
关键词
Raman spectroscopy; Confocal microscopy; Biofilms; Bacteria; Dental plaque
资金
- National Institutes of Health (NIH) [1-R21-DE016111-01A1, DE0-17157, DE0-13683]
- Department of Defense's (DoD's) National Defense Science & Engineering (NDSEG) program
- National Science Foundation's Graduate Research Fellowship Program (NSF GRFP)
A new method of mapping multiple species of oral bacteria in intact biofilms has been developed, using the optical technique of confocal Raman microscopy. A species classification algorithm, developed on dried biofilms, was used to analyze spectra of hydrated biofilms containing two microbial species central to dental health: Streptococcus sanguinis and Streptococcus mutans. The algorithm transferred successfully to the hydrated environment, correctly identifying the species of origin of single-species biofilms. We then used the algorithm successfully both to detect the presence of two species in mixed biofilms and to create spatial maps within these biofilms.
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