期刊
ADVANCED SCIENCE
卷 5, 期 10, 页码 -出版社
WILEY
DOI: 10.1002/advs.201800614
关键词
biooptical sensors; cell-free nucleic acids; circulating tumor DNA; liquid biopsy; microfluidics platforms
资金
- Ministry of Science, ICT, and Future Planning (MSIP) through the National Research Foundation of Korea (NRF) [2017R1A2B4005288]
- Korea Health Technology R & D Project through the Korea Health Industry Development Institute (KHIDI) - Ministry of Health & Welfare, Republic of Korea [HI16C-0272-010016]
- Asan Institute for Life Sciences, Asan Medical Center, Seoul, Republic of Korea [2017-7031, 7034]
- Korea Health Promotion Institute [HI16C0272010018] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2017R1A2B4005288, 2018H1A2A1059997] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Cell-free nucleic acids (cfNAs) are emerging diagnostic biomarkers for monitoring the treatment and recurrence of cancers. In particular, the biological role and clinical usefulness of cfNAs obtained from the plasma of patients with various cancers are popular and still intensely explored, yet most studies are limited by technical problems during cfNA isolation. A dimethyl dithiobispropionimidate (DTBP)-based microchannel platform that enables spontaneous cfNA capture in 15 min with minimal cellular background and no requirements for use of bulky instruments is reported first. This platform identified KRAS and BRAF hot-spot mutations following cfDNA isolation from the blood plasma and tissues obtained from 30 colorectal cancer patients. The correlation of mutations between the primary tissues and plasma from the patients was high using this platform with whole genome sequencing compared to the spin-column method. This platform can also be combined with various detection approaches (biooptical sensor, Sanger sequencing, and polymerase chain reaction (PCR)) for rapid, simple, low-cost, and sensitive circulating tumor DNA detection in blood plasma. The efficiency and versatility of this platform in isolating cfNAs from liquid biopsies has applications in cancer treatment and precision medicine.
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