期刊
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
卷 70, 期 -, 页码 206-210出版社
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S2053230X13034432
关键词
-
资金
- Cancer Center Support Grant [P30CA036727]
- National Institute of General Medical Sciences (NIGMS) [R01 GM101167, R01 GM082923]
- Nebraska Department of Health and Human Services [LB506]
- NIGMS [P41 GM103403]
- US DOE [DE-AC02-06CH11357]
Human primase synthesizes RNA primers and transfers them to the active site of Pol alpha with subsequent extension with dNTPs. Human primase is a heterodimer of two subunits: a small catalytic subunit (p49) and a large subunit (p58). The structural details of the initiation and elongation steps of primer synthesis, as well as primer length counting, are not known. To address these questions, structural studies of human primase were initiated. Two types of crystals were obtained. The best diffracting crystals belonged to space group P1, with unit-cell parameters a = 86.2, b = 88.9, c = 94.68 angstrom, alpha = 93.82, beta = 96.57, gamma = 111.72 degrees, and contained two heterodimers of full-length p49 and p59 subunits in the asymmetric unit.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据