期刊
3 BIOTECH
卷 1, 期 4, 页码 255-259出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s13205-011-0032-6
关键词
Bioethanol; Thermomyces lanuginosus; Thermophilic fungi; Xylanase; Xylose
资金
- Department of Agriculture, Government of Jharkhand
- CSIR [09/554(0017)/2008 EMR-I]
Thermomyces lanuginosus SS-8 was isolated from soil samples that had been collected from near self-heating plant material and its extracellular cellulase-free xylanase purified approximately 160-fold using ion exchange chromatography and continuous elution electrophoresis. This xylanase was thermoactive (optimum temperature 60 degrees C) at pH 6.0 and had a molecular weight of 23.79 kDa as indicated by SDS-PAGE electrophoresis. The xylanase rapidly hydrolyzed xylan directly to xylose without the production of intermediary xylo-oligosaccharides within 15 min of incubation under optimum conditions. This trait of rapidly degrading xylan to xylose as a sole end-product could have biotechnological potential in degradation of agro-wastes for bioethanol manufacturing industry.
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