期刊
STEM CELL REPORTS
卷 3, 期 1, 页码 -出版社
CELL PRESS
DOI: 10.1016/j.stemcr.2014.04.018
关键词
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资金
- MRC [MR/K008757/1]
- NSFC [81261130312]
- Natural Science Foundation of Guangdong Province, China [S2012010009414, S2012010009605]
- University Young Scholars Overseas Visiting Foundation of Guangdong Province
- RCUK fellowship
- MRC PhD studentships
- MRC [MR/K017047/1, MR/K008757/1, MC_U130184144, MC_UU_12014/2] Funding Source: UKRI
- Medical Research Council [1202171, MR/K017047/1, MR/K008757/1, MC_UU_12014/2, 974655, MC_U130184144] Funding Source: researchfish
In this study, human embryonic stem cell-derived hepatocytes (hESC-Heps) were investigated for their ability to support hepatitis C virus (HCV) infection and replication. hESC-Heps were capable of supporting the full viral life cycle, including the release of infectious virions. Although supportive, hESC-Hep viral infection levels were not as great as those observed in Huh7 cells. We reasoned that innate immune responses in hESC-Heps may lead to the low level of infection and replication. Upon further investigation, we identified a strong type III interferon response in hESC-Heps that was triggered by HCV. Interestingly, specific inhibition of the JAK/STAT signaling pathway led to an increase in HCV infection and replication in hESC-Heps. Of note, the interferon response was not evident in Huh7 cells. In summary, we have established a robust cell-based system that allows the in-depth study of virus-host interactions in vitro.
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