期刊
STEM CELL REPORTS
卷 3, 期 4, 页码 566-573出版社
CELL PRESS
DOI: 10.1016/j.stemcr.2014.08.002
关键词
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资金
- Spanish Ministry of Education and Science (MEC) [SAF2010-19498]
- Red Tematica de investigacion cooperativa en envejecimiento y fragilidad (RETICEF) [ISCIII2012-RED-43-029]
- Conselleria d'Educacio, Cultura i Esport de la Generalitat Valenciana [PROMETEO2010/074]
- INCLIVA [RS2012-609]
- EU [CM1001, FRAILOMIC-HEALTH.2012.2.1.1-2]
- FEDER from the European Union
High rates of stem cell proliferation are important in regenerative medicine and in stem cell banking for clinical use. Ambient oxygen tensions (21% O-2) are normally used for in vitro culture, but physiological levels in vivo range between 3% and 6% O-2. We compared proliferation of human dental pulp stem cells (hDPSCs) cultured under 21% versus 3% O-2. The rate of hDPSC proliferation is significantly lower at 21% O-2 compared to physiological oxygen levels due to enhanced oxidative stress. Under 21% O-2, increased p38 phosphorylation led to activation of p21. Increased generation of reactive oxygen species and p21 led to activation of the NRF-2 signaling pathway. The upregulation of NRF-2 antioxidant defense genes under 21% O-2 may interact with cell-cycle-related proteins involved in regulating cell proliferation. Activation of p38/p21/NRF-2 in hDPSCs cultured under ambient oxygen tension inhibits stem cell proliferation and upregulates NRF-2 antioxidant defenses.
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