期刊
STEM CELL REPORTS
卷 1, 期 2, 页码 198-208出版社
CELL PRESS
DOI: 10.1016/j.stemcr.2013.07.001
关键词
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资金
- Toyobo Biotechnology Foundation
- Uehara Memorial Foundation
- Human Frontier Science Program
- Japan Society for the Promotion of Science
- US National Institutes of Health [R01 CA086065, U01 HL099999]
- California Institute for Regenerative Medicine [RT2-02060]
- Leukemia & Lymphoma Society [700709]
Very small embryonic-like stem cells (VSELs) isolated from bone marrow (BM) have been reported to be pluripotent. Given their nonembryonic source, they could replace blastocyst-derived embryonic stem cells in research and medicine. However, their multiple-germ-layer potential has been incompletely studied. Here, we show that we cannot find VSELs in mouse BM with any of the reported stem cell potentials, specifically for hematopoiesis. We found that: (1) most events within the VSEL'' flow-cytometry gate had little DNA and the cells corresponding to these events (2) could not form spheres, (3) did not express Oct4, and (4) could not differentiate into blood cells. These results provide a failure to confirm the existence of pluripotent
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