期刊
NANOTECHNOLOGY REVIEWS
卷 3, 期 4, 页码 389-400出版社
WALTER DE GRUYTER GMBH
DOI: 10.1515/ntrev-2013-0041
关键词
heat shock proteins; mitochondrial transmembrane potential; nanomedicine; nanoparticles; role of caspases; selective tumoricidal activity
类别
资金
- NSF [HRD0450375, HRD1043316]
- NIH-supported INBRE program of NCRR [P20RR016456]
- NIH [1RO1CA142-01A1]
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1230357] Funding Source: National Science Foundation
- Division Of Human Resource Development
- Direct For Education and Human Resources [1043316] Funding Source: National Science Foundation
The hyperthermia-induced cytotoxicity of gold-coated SPIONs conjugated to LHRH (SPIONs@Au-LHRH) has been studied in LHRH-receptor overexpressing murine GT1-7 hypothalamic neurons (noncancerous) and human LNCaP cells (cancerous). In the absence of an external magnetic field, SPIONs@Au-LHRH were least cytotoxic to either cell type. When cells were pretreated with SPIONs@Au-LHRH and then exposed to a magnetic field (465 Oe for 15 or 2x15 min), both cell types showed marked decreases in viability and proliferation. The cell death in GT1-7 neurons was found to be late apoptosis or early necrosis, while necrosis was prominent in LNCaP cells. The LNCaP cells exposed to the magnetic field for 15 min showed a significant drop in the mitochondrial transmembrane potential; however, no such change was evident in GT1-7 neurons for the first 15 min. The cell death in LNCaP cells was found to be mediated through the caspase-3-dependent pathway. There was an increased expression of heat shock protein 70 in GT1-7 neurons, and no such increase was seen in LNCaP cells. It is suggested that noncancerous GT1-7 neurons are more resistant to the heat-induced cytotoxicity of SPIONs@Au-LHRH than are LNCaP cells due to increased expression of HSP70. The results show promise toward the selective tumoricidal actions of targeted magnetic nanoparticles.
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