期刊
MICROBIOLOGYOPEN
卷 3, 期 6, 页码 897-909出版社
WILEY
DOI: 10.1002/mbo3.214
关键词
Biofilm; proteases; Staphylococcus aureus; sarA
类别
资金
- National Institute of Allergy and Infectious Disease [R56-AI074935, R56-AI093126]
- Department of Defense U.S. Army Medical Research and Materiel Command [W81XWH-10-1-0991, W81XWH-12-2-0020]
- Translational Research Institute through NIH National Center for Research Resources [UL1TR000039]
- National Center for Advancing Translational Sciences
- Center for Microbial Pathogenesis and Host Inflammatory Responses [P20-GM103450]
- NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES [UL1TR000039] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R56AI074935, R01AI074935, R01AI119380, R56AI093126] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P30GM103450, P20GM103625] Funding Source: NIH RePORTER
We demonstrate that the purified Staphylococcus aureus extracellular proteases aureolysin, ScpA, SspA, and SspB limit biofilm formation, with aureolysin having the greatest impact. Using protease-deficient derivatives of LAC, we confirmed that this is due to the individual proteases themselves. Purified aureolysin, and to a lesser extent ScpA and SspB, also promoted dispersal of an established biofilm. Mutation of the genes encoding these proteases also only partially restored biofilm formation in an FPR3757 sarA mutant and had little impact on restoring virulence in a murine bacteremia model. In contrast, eliminating the production of all of these proteases fully restored both biofilm formation and virulence in a sarA mutant generated in the closely related USA300 strain LAC. These results confirm an important role for multiple extracellular proteases in S. aureus pathogenesis and the importance of sarA in repressing their production. Moreover, purified aureolysin limited biofilm formation in 14 of 15 methicillin-resistant isolates and 11 of 15 methicillin-susceptible isolates, while dispersin B had little impact in UAMS-1, LAC, or 29 of 30 contemporary isolates of S. aureus. This suggests that the role of sarA and its impact on protease production is important in diverse strains of S. aureus irrespective of their methicillin resistance status.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据