期刊
METHODS AND APPLICATIONS IN FLUORESCENCE
卷 3, 期 4, 页码 -出版社
IOP PUBLISHING LTD
DOI: 10.1088/2050-6120/3/4/042006
关键词
FRET; energy transfer; intracellular; detection
资金
- Office of Naval Research
- Naval Research Laboratory Nanosciences Institute
- DTRA JSTO MIPR [B112582M]
- NRL
Probes that exploit Forster resonance energy transfer (FRET) in their feedback mechanism are touted for their sensitivity, robustness, and low background, and thanks to the exceptional distance dependence of the energy transfer process, they provide a means of probing lengthscales well below the resolution of light. These attributes make FRET-based probes superbly suited to an intracellular environment, and recent developments in biofunctionalization and expansion of imaging capabilities have put them at the forefront of intracellular studies. Here, we present an overview of the engineering and execution of a variety of recent intracellular FRET probes, highlighting the diversity of this class of materials and the breadth of application they have found in the intracellular environment.
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