期刊
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
卷 -, 期 88, 页码 -出版社
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/51649
关键词
Infection; Issue 88; Zebrafish; Staphylococcus epidermidis; Mycobacterium marinum; automated injection; high throughput screening; COPAS XL; VAST BioImager; host pathogen interaction; drug screen; CLSM
资金
- BioMedical Materials institute [P5.03 IBIZA]
- Dutch Ministry of Economic Affairs
- Netherlands Ministry of Economic Affairs [NWOA_6QY9BM]
- Netherlands Ministry of Education, Culture and Science
- EU [FP7-Health-2009-242048]
- EU Initial Training Network FishForPharma [PITN-GA-2011-289209]
- Innovative Medicines Initiative Joint Undertaking [115337]
- European Union
- Leiden University Fund (LUF) for robotics
- Cyttron, in the Besluit Subsidies Investeringen Kennisinfrastructuur program
- Netherlands Organization for Scientific Research
- Division for Earth and Life Sciences (ALW)
- Netherlands Organization for Scientific Research (NWO) [834.10.004]
Zebrafish are becoming a valuable tool in the preclinical phase of drug discovery screenings as a whole animal model with high throughput screening possibilities. They can be used to bridge the gap between cell based assays at earlier stages and in vivo validation in mammalian models, reducing, in this way, the number of compounds passing through to testing on the much more expensive rodent models. In this light, in the present manuscript is described a new high throughput pipeline using zebrafish as in vivo model system for the study of Staphylococcus epidermidis and Mycobacterium marinum infection. This setup allows the generation and analysis of large number of synchronous embryos homogenously infected. Moreover the flexibility of the pipeline allows the user to easily implement other platforms to improve the resolution of the analysis when needed. The combination of the zebrafish together with innovative high throughput technologies opens the field of drug testing and discovery to new possibilities not only because of the strength of using a whole animal model but also because of the large number of transgenic lines available that can be used to decipher the mode of action of new compounds.
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