期刊
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
卷 -, 期 75, 页码 -出版社
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/50172
关键词
Medicine; Issue 75; Biomedical Engineering; Anatomy; Physiology; Biophysics; Pathology; lung diseases; asthma; respiratory function tests; respiratory system; forced oscillation technique; respiratory system mechanics; airway hyperresponsiveness; flexiVent; lung physiology; lung; oxidative stress; ventilator; cannula; mice; animal model; clinical techniques
资金
- Canadian Thoracic Society
The forced oscillation technique (FOT) is a powerful, integrative and translational tool permitting the experimental assessment of lung function in mice in a comprehensive, detailed, precise and reproducible manner. It provides measurements of respiratory system mechanics through the analysis of pressure and volume signals acquired in reaction to predefined, small amplitude, oscillatory airflow waveforms, which are typically applied at the subject's airway opening. The present protocol details the steps required to adequately execute forced oscillation measurements in mice using a computer-controlled piston ventilator (flexiVent; SCIREQ Inc, Montreal, Qc, Canada). The description is divided into four parts: preparatory steps, mechanical ventilation, lung function measurements, and data analysis. It also includes details of how to assess airway responsiveness to inhaled methacholine in anesthetized mice, a common application of this technique which also extends to other outcomes and various lung pathologies. Measurements obtained in naive mice as well as from an oxidative-stress driven model of airway damage are presented to illustrate how this tool can contribute to a better characterization and understanding of studied physiological changes or disease models as well as to applications in new research areas.
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