期刊
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
卷 -, 期 77, 页码 -出版社
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/50575
关键词
Bioengineering; Issue 77; Molecular Biology; Biomedical Engineering; Chemistry; Biochemistry; Biophysics; Physics; Cellular Biology; Molecular Imaging; Mass Spectrometry; MS; MSI; Desorption electrospray ionization; DESI; Ambient mass spectrometry; tissue; sectioning; biomarker; imaging
资金
- ARRA NSF MRI Instrument Development grant [0923179]
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [0923179] Funding Source: National Science Foundation
Mass spectrometry imaging (MSI) provides untargeted molecular information with the highest specificity and spatial resolution for investigating biological tissues at the hundreds to tens of microns scale. When performed under ambient conditions, sample pre-treatment becomes unnecessary, thus simplifying the protocol while maintaining the high quality of information obtained. Desorption electrospray ionization (DESI) is a spray-based ambient MSI technique that allows for the direct sampling of surfaces in the open air, even in vivo. When used with a softwarecontrolled sample stage, the sample is rastered underneath the DESI ionization probe, and through the time domain, m/z information is correlated with the chemical species' spatial distribution. The fidelity of the DESI-MSI output depends on the source orientation and positioning with respect to the sample surface and mass spectrometer inlet. Herein, we review how to prepare tissue sections for DESI imaging and additional experimental conditions that directly affect image quality. Specifically, we describe the protocol for the imaging of rat brain tissue sections by DESI-MSI.
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